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Chunk #24 — Methods — Stereotaxic virus injection

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Selective activation of cholinergic interneurons enhances accumbal phasic dopamine release: setting the tone for reward processing.
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An AAV-ChR2 vector was injected bilaterally (500 nl/side) into the nucleus accumbens of mice that were allowed to recover for 4 - 8 weeks after virus injection, before any subsequent intervention.