Because differential absorption, distribution, or clearance of ethanol may have contributed to the increased righting response time observed in Lwt/+ mice, blood ethanol concentrations (BEC) were measured for 180 minutes after a single administration of ethanol (3.6 g/kg, i.p.). A parallel experiment was also performed to control for longitudinal sampling, where a single blood sample was obtained from a separate cohort at t = 180 minutes. Importantly, we did not observe a difference in BEC at any timepoint between Lwt/+ mice and wild-type littermates [F1,48(genotype) = 0.63, P = 0.44] (Figure 5B).
