Nanog-Luciferase ESCs31 were infected and measured after 8 days. Hits were identified if they reduced luciferase levels (z<−6) across all replicates and did not reduce AlamarBlue levels. Hits were validated in wild-type ESCs by measuring mRNA levels of Oct4, Nanog, Sox2, Klf4, and Zfp42. Oct4 expression was assessed using immunofluorescence staining and morphology was visually assessed.
