Hippocampal samples (total hippocampus) from selectively bred alcohol-preferring (P) and non-preferring (NP) rats, generation S70, were obtained from the Indiana University School of Medicine. The P and NP lines of rats were derived from a randomly bred, closed colony of Wistar rats by mass selection using a two-bottle free-choice paradigm with access to 10% (V/V) ethanol and water [25]. The sample consisted of eight alcohol preferring (P) and eight non-preferring (NP) male alcohol-naïve rats, all sacrificed at 90 days of age. For further details see [26].
