The 3xTg-AD mice are a well-validated “humanized” animal model that uniquely expresses plaques, tangles, and associated cellular and brain-regional pathologies associated with Alzheimer’s disease (Oddo, Caccamo, Shepherd, et al., 2003). The 3xTg-AD mice were derived single-cell embryos from mice bearing a knock-in mutation of human presenilin (PS1M146V) on a mixed C7BL/6;129X1/SvJ;129S1/Sv genetic background (B6;129-PSEN-1tm1Mpm). These mice were co-injected with two mutant human transgenes for amyloid beta precursor protein (APPSwe) and microtubule-associated protein Tau (TauP30IL). Both transgenes integrated at the same locus.
