1M PBMCs from each donor were stained using standard procedure (30 min, 4 C) with the following surface antibody panel (CD3-PerCP clone SK7 (BioLegend), CD4-APC clone OKT4 (BioLegend), CD8-BV570 clone RPA-T8 (BioLegend), CD14-FITC clone 63D3 (BioLegend), CD19-BV510 clone SJ25C1 (BD), and Ghost dye A710 viability stain (Tonbo)) (Life Sciences Reporting Summary). Samples were then analyzed and sorted using a BD FACSAria Fusion instrument at the UCSF flow cytometry core. To calculate cell type proportions, the number of events in each of CD3+ CD4+ CD8− (CD4+ T cells), CD3+ CD4− CD8+ (CD8+ T cells), CD3− CD19+ (B cells), and CD3− CD14+ (monocytes) were divided by the sum of events in these gates (Supplementary Fig. 21).
