RNA was isolated from sorted CD4+ T cells following the RNeasy micro kit protocol (QIAGEN), and cDNA was prepared using MultiScribe Reverse Transcriptase (Applied Biosystems cat #4368814). The qPCR primers were chosen from the PrimerBank reference when available 50. Each sample was run in triplicate with the Luminaris HiGreen qPCR kit (Thermo Scientific #K0992) according to standard protocol using a Roche Light Cycler 96 instrument and fold change was calculated from ΔΔCT between control and stimulated samples with GAPDH as a reference gene.
