Cellular suspensions were loaded onto the 10x Chromium instrument (10x Genomics) and sequenced as described in Zheng et al17. The cDNA libraries were sequenced using a custom program on 10 lanes of Illumina HiSeq2500 Rapid Mode, yielding 1.8B total reads and 25K reads per cell. At these depths, we recovered >90% of captured transcripts in each sequencing experiment.
