The extracellular domains of mouse LPHN3 and mouse FLRT3 and their shorter constructs were cloned as C-terminal Fc-fusion proteins into a modified pCMV6-XL4 plasmid and were expressed as soluble entities in the cell culture medium of stably transfected HEK293 GnTI- cells and maintained in 10%FBS in Dulbecco’s Modified Eagle Medium (DMEM).
