Proteins were affinity purified using Protein-A resin using in 50mM TrisHCl pH 7.4, 150mM NaCl, 1 mM EDTA (TNE), and subsequently cleaved with 3C protease to remove the Fc fragment. Proteins were further purified by size exclusion chromatography in 50mM Tris-HCl pH 7.4 and 150mM NaCl (TN) and concentrated depending of the downstream experiment.
