Experiments were performed with a MicroCaliTC200 system, purified proteins were buffer-exchanged in 50 mM Na-HEPES pH 7.0, 150 mM NaCl, 1 mM EDTA (or 2mM CaCl2). The protein solution in the syringe was added to the cell in a series of multiple injections at 25°C. The raw ITC data was processed and fitted using a single site model using the ORIGIN software provided by GE MicroCal.
