Furthermore, to compare the response of FoxO3a-deficient mice versus comparable wild type mice to antidepressant, a group of homozygous FoxO3a-deficient and littermate wild-type mice received daily injection of saline or imipramine (20 mg/kg/day, i.p.) for 23 days. When tested in the FST, there was a significant difference in immobility among the four groups of mice with different genotypes and treatments (Figure 5e). When multiple comparison with Bonferroni correction was conducted using the wild type saline-treated mice as control, imipramine significantly reduced immobility in wild type mice (p<0.0018), and FoxO3a-defidient mice, either without or with imipramine treatment, also exhibited reduced immobility (p<0.0019 and p<0.0001, respectively). On the other hand, when using the FoxO3a-deficient mice with saline treatment as control, the immobility in imipramine-treated mice, either wild type or FoxO3a-deficient, was not different from control.
