Chunk #8 — Materials and methods — Liver pathology — Paraffin processing of mice livers tissue

Source: High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2.
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Liver tissue was fixed in10% neutral formalin, dehydrated in graded ethanol solutions (70, 80, 90, 95, and 100%) at 4°C and embedded in paraffin at 60°C.