In the CN, 12 genes showed both differential methylation and differential gene expression. DE statistics, EWAS summary statistics and functional annotation for these genes are provided in Supplementary Table S11. No overlap was observed in the VS and PUT. At the module-level, co-expression module “e-CN-magenta” showed significant overlap with the methylation modules “m-CN-red” (p = 0.003) and “m-CN-midnightblue” (p = 0.014) (Fig. 2C), while expression module “e-CN-purple” did not show significant overlap with the methylation modules in CN. Of the 3 AUD-associated expression modules in the VS, only “e-VS-salmon” showed significant overlap with the methylation module “m-VS-turquoise” (p = 0.003), but not “m-VS-lavender”. No overlap was observed for gene expression and DNA-methylation in PUT.
