Chunk #34 — Methods — Gene expression validation by qRT-PCR

Source

Characteristic analyses of a neural differentiation model from iPSC-derived neuron according to morphology, physiology, and global gene expression pattern.

Embedded

yes

Text

Total RNA was reverse transcribed into cDNA using the PrimeScriptTMRT reagent Kit with gDNA Eraser (Perfect Real Time). Real-time quantitative PCR was performed with a SYBR® Premix Ex TaqTM II detection System. The primer sequences are shown as follows: