Chunk #33 — Methods — Cell collection and mRNA preparation

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Characteristic analyses of a neural differentiation model from iPSC-derived neuron according to morphology, physiology, and global gene expression pattern.

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Cells were collected at different time points. Total RNA was extracted using RNeasy mini kit, in combination with RNAase-free DNAase to remove the potential genomic DNA contamination (Qiagen). RNA concentration was quantified by Nanodrop 2000C Spectrophotometer.