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Chunk #7 — Materials and Methods — Chronic Ethanol

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Gene expression in brain and liver produced by three different regimens of alcohol consumption in mice: comparison with immune activation.
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The Chronic ethanol treatment group included 10 each treated and control mice maintained on a 12∶12 light/dark light cycle (lights on at 07∶00 AM). Mice were approximately 3 months old at the beginning of experiments. Water and ethanol were continuously available for a 30-day period and bottle positions were changed daily to control for position preference. Control animals received only water.