56Rs910083 resides in an active promoter marked by H3K9ac in several brain regions examined in the Roadmap Epigenomics Project,56 and it associates with DNMT3B RNAexp specifically in the cerebellum. This finding does not negate the importance of other brain regions known to be involved in SUDs but brings up the possibility of altered gene regulation in the cerebellum contributing to the complex neurobiological pathway leading to dependence. A main function of the cerebellum is motor coordination, but it is also involved in non-motor functions relevant to SUD, including reward.46–48 The cerebellum responds to acute and long-term exposures to nicotine57–62 and other substances,47 and it makes functional connections with the prefrontal cortex and other brain tissues that are widely recognized for their involvement in SUDs.47
