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Chunk #29 — Methods — Food intake and re-feeding experiments

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FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase.
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Male mice (8–10 weeks old, body weight-matched) were housed individually and provided food (NC or HFD) and water ad libitum. The food intake was daily recorded at 8:00 and 17:00 hours during a 7-day period and was normalized to initial body weight. For the re-feeding experiments, mice were fasted overnight for 18 h with water provided ad libitum. The re-feeding food intake, body weight and blood glucose were recorded at the indicated time points.