To establish the subject’s LR, they were scheduled for a laboratory-based alcohol challenge (>90% of those invited agreed to participate). Here, the subjects were required to have zero baseline breath alcohol concentrations (BrACs), after which they drank alcohol (0.75 ml/kg for males and 0.70 for females) given as a 20% by volume solution in a room temperature carbonated beverage and consumed from a closed thermos over 10 minutes to produce approximately equivalent BrACs across genders [41–43]. The initial alcohol challenge and fMRI sessions were scheduled on non-consecutive days, but within a week of each other whenever possible. During the initial alcohol challenge session, at 15 minutes, and every 30 minutes over 180 minutes participants completed the Subjective High Assessment Scale (SHAS) and BrAC evaluations, but reflecting restrictions in both testing time and finances, no other biological components were evaluated. The SHAS consists of 13 items graded on a 39-point scale to evaluate alcohol effects [43]. Subjects then participated in two fMRI sessions, receiving in random order alcohol (the same dose as in the prior session) or placebo, entering the scanner
