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Chunk #40 — Materials and methods — Morris Water Maze

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Alcohol drinking exacerbates neural and behavioral pathology in the 3xTg-AD mouse model of Alzheimer's disease.
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For assessment of spatial memory, a probe trial was conducted 1h after the final acquisition trial. During the probe trial, mice were placed in the pool for 60s in the absence of the platform but with extra-maze cues in place. Time in each quadrant of the pool and the number of crossings of the target location (where the platform was located during training) was assessed and used to demonstrate memory of the platform location. Spatial memory was demonstrated by a preference for the quadrant where the platform had been located, in comparison to other quadrants of the pool.