For acquisition of spatial learning, mice were tested for the ability to find a submerged escape platform with the use of spatial cues. During this stage, extra-maze cues were evenly placed around the pool to divide it into quadrants. Mice were given four trials daily until they reached criterion for learning (<15s to find the platform). If a mouse failed to locate the platform in 60s, it was placed on the platform for 10s before being removed and allowed to rest (30s inter-trial interval) before starting the next trial. Spatial learning was demonstrated by a decrease in the latency to find the escape platform and increased efficiency in the path taken to reach the platform.
