Brains were dissected from E13 rat embryos. Paraffin embedded brains were cut on a cryostat. Cortical sections were taken and immunohistochemistry was performed. Sections were stained using antibodies against ZNF804A (1∶500; Santa Cruz Biotechnology) with a nuclear counter stain TO-PRO (Invitrogen, T-3605). Anti-rabbit 488 secondary antibody (1∶400, Molecular Probes) was used for fluorescent imaging. Images were taken with a Leica TCS SP2 confocal microscope.
