RNA (1 µg) was reverse-transcribed into cDNA using oligo-dT primers and reverse-transcriptase. RNA was then hydrolyzed, and re-suspended in nuclease-free water. Gene-specific primers were designed using Primer 3 software (http://frodo.wi.mit.edu/primer3/) and tested for efficiency and specificity by serial dilutions and melt curve analysis. Sybr Green mix (ABI) was used to amplify cDNA. Primer sequences are in Table S1.
