In a two-bottle, free-choice paradigm in which mice could drink either water or an ascending series of ethanol concentrations, pre-treatment with LPS significantly increased the amount of ethanol consumed in B6 male mice after one injection of LPS as well as after two LPS injections (Fig. 2A and D). A similar increase of alcohol intake was seen in both experimental groups after the first period (one week) of alcohol deprivation (Fig. 2B and E). However, after a second period (one month) of alcohol deprivation, a slight increase of alcohol intake still was seen only in B6 male mice after one injection of (Fig. 2C and F). Complete data for ethanol intake (amount of ethanol consumed, preference for ethanol, and total fluid intake) in a continuous-access, two-bottle choice test for B6 male mice are presented in Supplemental Figs. 1 and 2 (for detailed statistics see Supplementary materials in Supplemental Table 1).
