Results for females were similar to those for males; pretreatment with LPS increased ethanol consumption in B6 female mice after one injection of LPS as well as after two LPS injections (Fig. 3A and D). However, after one week of alcohol deprivation the increased alcohol intake was observed only for mice given two injections of LPS (Fig. 3B and E). After one month of alcohol deprivation, the increased alcohol intake was seen more clearly in B6 female mice after two injections of LPS than after one injection of LPS (Fig. 3C and F). The schedule for two injections of LPS and testing of drinking as well as the effects of LPS on body weight and water consumption are shown in Fig. 1. LPS produced a marked decrease in body weight and water consumption which was more pronounced for the first injection compared with the second. Complete data for ethanol intake (amount of ethanol consumed, preference for ethanol, and total fluid intake) in a continuous-access, two-bottle choice test for B6 female mice are presented in Supplemental Figs. 3 and 4 (for detailed statistics see Supplementary materials in Supplemental Table 2).
