A separate group of 20 male P rats were each provided access to a saccharin solution (0.04 g/L) during a 2-h daily access period (1100–1300 hours, beginning one hour after lights out) 5 days/week for 16 days until saccharin intake stabilized (as determined by 2-way ANOVA with repeated measures on days 13–16, with no significant effect of day on saccharin intake). Food and water were freely available for 24 hours/day throughout this study. Saccharin and water intakes were recorded daily and body weights were recorded twice weekly. The rats were 4 months old and had a mean body weight of 455 g at the start of clonidine treatment.
