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Chunk #12 — Materials and methods — Drugs — Study 2: effect of clonidine treatment on saccharin intake

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The α2-adrenergic receptor agonist, clonidine, reduces alcohol drinking in alcohol-preferring (P) rats.
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The 0.04 g/L saccharin solution was prepared by dissolving 99+% saccharin (Sigma-Aldrich Co., St. Louis, MO) in distilled, deionized water. Saccharin and water were presented in calibrated glass drinking tubes, and daily fluid intakes were recorded to the nearest mL. Saccharin and water intakes are reported in mL/kg BW to control for size-based variation in fluid requirements/gastric capacity.