The RAW264.7 mouse macrophage cell line was transiently transfected with iNOS- or TNFα-promoters directing luciferase expression (Pascual et al., 2005) using SuperFect (Qiagen). Double transfections with plasmid DNA and siRNAs used Transmessenger reagent (Qiagen). Flag-tagged full-length (FL) mouse Nurr1 was cloned into p3XFLAG-CMV-7.1 vector (Sigma). Mutant constructs of Nurr1 were generated with the Quick-change site-direct mutagenesis kit (Stratagene). Retrovirus production (Openbiosystems) and infection into BV2 cells were performed according to the manufacturer’s protocol. Lentivirus p156RRLsinPPTCMV-GFP-PREU3Nhe and pHAGE vector packaging was done using Virapower (Invitrogen). GIPZ lentivirus shRNAmir (Openbiosystems) packaging was performed according to the manufacturer’s protocols. See Supplemental Experimental procedures for full methods.
