Cell death detection kit (Roche) was used for TUNEL assay. SUMOylation assays were performed as described before (Pascual et al., 2005). For endogenous co-IP experiments, anti-Nurr1 (E-20, Santa Cruz), anti-CoREST (E-15, Santa Cruz and Millipore) and anti-p65 (C-20, Santa Cruz) were used for IP and Western blotting. For immunoprecipitation of tagged proteins, M2 anti-Flag-agarose (Sigma) beads were used. NLK (Millipore) in vitro kinase reaction (Kanei-Ishii et al., 2004) was performed using GST-fusion substrates.
