For each experimental condition, 2 × 107 BV2 cells or 6 × 106 mouse primary astrocytes were used. Cells were stimulated with LPS for BV2 cells and IL1β for astrocytes for the indicated time before crosslinking for 10 minutes with 1% formaldehyde. Anti-Nurr1 (E- 20, Santa Cruz Biotechnology) anti-p65 (C-20, Santa Cruz Biotechnology), anti-CoREST (Millipore) or control rabbit IgG (Santa Cruz Biotechnology) were used for IP. See details in Supplemental Experimental Procedures.
