Chunk #42 — EXPERIMENTAL PROCEDURES — In vivo birth-dating, genetic manipulation of neural progenitors with engineered retroviruses and pharmacological treatments
For pharmacological treatments, rapamycin (LC Laboratories) was first dissolved in ethanol at a stock concentration of 25 mg/ml and was further diluted with a final concentration of 1 mg/ml in 4 % ethanol, 5% Tween 80, and 5% PEG 400 as previously described (Zhou et al., 2009). Rapamycin was intraperitoneally delivered to mice (20 mg/kg body weight). SB-216763 (Tocris bioscience) was dissolved in DMSO at a stock concentration of 75 mM and intraperitoneally delivered to mice (2 mg/kg body weight) daily during the course of experiments (Mao et al., 2009).
