Chunk #41 — EXPERIMENTAL PROCEDURES — In vivo birth-dating, genetic manipulation of neural progenitors with engineered retroviruses and pharmacological treatments
Adult female C57BL/6 mice (7-8 weeks old) housed under standard conditions were anaesthetized and concentrated retroviruses were stereotaxically injected into the dentate gyrus at 4 sites (0.5 μl per site at 0.25 μl/min) with the following coordinates (posterior = 2 mm from Bregma, lateral = ± 1.6 mm, ventral = 2.5 mm; posterior = 3 mm from Bregma, lateral = ± 2.6 mm, ventral = 3.2 mm) as previously described (Duan et al., 2007). In one set of experiments (Figure S10), adult female 129S6 mice were used. All animal procedures used in this study were performed in accordance with the protocol approved by the Institutional Animal Care and Use Committee at Johns Hopkins University School of Medicine.
