Exposure to ethanol vapor in ethanol sedation assays causes dose-dependent sedation and internal ethanol concentrationsData are from w1118 control female (A, C and E) and male (B, D and F) flies exposed to vapor from the indicated concentrations of ethanol (0, 30, 40 and 50%). A and B. Ethanol sedation time-course. Time and ethanol concentration had significant effects on percent active flies and there was a significant interaction between time and ethanol concentration for both females and males (individual two-way ANOVAs; time, p<0.0001; ethanol concentration, p<0.0001; interaction, p<0.0001; n=5 for females, n=10 for males). C and D. Ethanol sedation ST50 values. ST50 values derived from the data in panels A and B were significantly affected by ethanol concentration in both males and females (individual one-way ANOVAs, p<0.0001, n=5 for females, n=10 for males). ST50 values in response to all ethanol concentrations were significantly different (Bonferroni’s multiple comparison, p<0.001 in all cases). ST50 values cannot be calculated for flies exposed to 0% ethanol (water) because flies do not become sedated in the absence of the drug. E and F. Internal ethanol concentrations. A 60-minute exposure to vapor from increasing concentrations of ethanol progressively increased whole body internal ethanol concentrations in flies (individual one-way ANOVAs, p≤0.0002, n=6 for females, n=5 for males). Internal ethanol after any given exposure was significantly different from internal ethanol in the next lower and higher groups (Bonferroni’s, p<0.05).
