Mini-w and endogenous w influence ethanol sensitivity measured in eRING assaysA. Flies harboring transposon insertions in TGFβR genes (tkv, wit and babo; blue circles), Akap200 (green triangles) or Gal4 drivers (red squares) were ranked by eye color (w+ rank, X axis) and tested in eRING assays for sedation to ethanol vapor from 30% ethanol (TGFβR and Gal4 drivers) or 50% ethanol (Akap200). Compiled T50 values (fold of w1118 controls) from all genotypes correlated with w+ rank (Pearson r=0.7503, p<0.0001). TGFβR lines tested were tkv alleles 7, 8, d07811, f02766, f03305, c06013 and KG05071, wit alleles d02492, e00566 and e01243 and babo alleles c04263, c05710, k16912. Akap200 lines tested were EP2254, c01373, d01782, d03938, d07255, EY04645 and EY12242. Gal4 lines tested were da-Gal4/+, mef2-Gal4/+, Appl-Gal4/+, Actin5cGal4/+, GMR-Gal4/+, 24B-Gal4/+, and elav-Gal4/+. See Figure S3 for representative eye color images. B. There was an overall effect of genotype on T50s from eRING studies using vapor from 30% ethanol (one-way ANOVA, p=0.0003, n=10 per genotype). mini-w-expressing elav-Gal4/+ and v30034/+ flies had elevated T50 values compared to white-eyed w1118 controls and elav-Gal4,v30034 white knockdown flies (*Bonferroni’s, p<0.05). T50s in w1118 controls and elav-Gal4,v30034 flies were not distinguishable (Bonferroni’s, n.s.). C. T50s in w1118 flies tested in eRING studies with vapor from 30% ethanol were significantly lower than in w+ flies (Kolmogorov-Smirnov test for Gaussian distribution; w1118, p>0.01, n.s.; w+, p=0.0017, significantly non-Gaussian; *Mann-Whitney test, p=0.0147, n=10).
