AB18465), TBR1 (rabbit, 1:200; Abcam: AB31940), TBR2 (rabbit, 1:300; Abcam: AB23345), BRN2 (mouse, 1:50; Millipore: MABD51), RELN (mouse, 1:200; MBL: D223-3). Alexa Fluor Dyes (LifeTechnologies) were used at 1:1,000 dilution for amplifying the signal. Nuclei were visualized with Hoechst 33258 (LifeTechnologies, 1:10,000). Cryosections were mounted for microscopy on glass coverslips, using Aquamount (ThermoScientific) and imaged on a Zeiss M1 Axioscope with a 10×, 20×, 40× or 63× objective. All cells expressing a particular marker were counted on sections and normalized to the total number of cells.
