Cryosections were washed with PBS to remove excess OCT and blocked in 10% normal goat serum (NGS), 0.1% bovine serum albumin (BSA), 0.3% Triton X-100 diluted in PBS for one hour at room temperature. The sections were then incubated overnight at 4 °C with primary antibodies diluted in solution containing 2% NGS and 0.1% Triton X-100. PBS was used to wash the primary antibodies and the cryosections were incubated with secondary antibodies containing 2% NGS and 0.1% Triton X-100 for 1 h. The following primary antibodies were used for immunocytochemistry: PAX6 (rabbit, 1:300; Covance: PRB-278P), PAX6 (mouse, 1:300; DSHB), p-vimentin (mouse, 1:2,000; Abcam: ab22651), N-CAD (mouse, 1:50; Santa Cruz: 8424), FOXG1 (rabbit, 1:500; NCFAB), NEUN (mouse, 1:500, Millipore: MAB377), MAP2 (guinea pig, 1:1,000; Synaptic Systems: 188004), GFAP (rabbit; 1:500; DAKO: Z0334), SATB2 (mouse, 1:50; Abcam: AB51502), CTIP2 (rat, 1:300; Abcam: AB18465), TBR1 (rabbit, 1:200; Abcam: AB31940), TBR2 (rabbit, 1:300; Abcam: AB23345), BRN2 (mouse, 1:50; Millipore: MABD51), RELN (mouse, 1:200; MBL: D223-3). Alexa Fluor Dyes (LifeTechnologies) were used at 1:1,000 dilution for amplifying the signal. Nuclei were visualized with Hoechst 33258
