Another less invasive method to obtain cells from peripheral blood is to isolate specific mononuclear cells. The isolation of these cells can be conducted via density gradient centrifugation. Finally, mature T-cells and myeloid cells are purified and can be used for further reprogramming. But, from the current stand of research, it is not known if iPSCs generated from peripheral blood T cells differentiate normally. They have preexisting V(D)J rearrangements at the T-cell receptor loci which could lead to the development of T-cell lymphomas [56]. Also, the reprogramming efficiency of these cells is ten to fifty times lower compared to human fibroblasts [57].
