A Clic4 co-expression network was initially generated using Pearson product-moment correlation coefficients with basal gene expression data from medial PFC across 27 BXD recombinant inbred mice, as well as C57BL/6J and DBA/2J progenitors (n = 29). This was done within the GeneNetwork resource for genetic analysis of genomic and phenotypic traits (www.genenetwork.org). To address possible false positives due to multiple comparisons, p-values were corrected using the q-value false-discovery rate method (Qian & Huang, 2005) within the R statistical framework (http://www.bioconductor.org/packages/2.4/bioc/html/qvalue.html), and resulting q-values were filtered for q<0.05, resulting in a total of 1015 probe sets. Ingenuity Pathway Analysis (www.ingenuity.com) was utilized to assess the biological significance and relationships between these genes, based on current scientific literature, using the Pearson correlation coefficients (positive and negative) as signals for individual genes (Figure S7).
