Flies were grown at 20°C (Clic homozygous mutants) or 25°C (all other genotypes) and 55% relative humidity on a standard sugar:yeast:cornmeal:agar medium (10%:2%:3.3%:1% w/v) supplemented with 0.2% Tegosept (Sigma Chemical Co., St. Louis, MO, USA) and active dry yeast under a 12-h light-dark cycle. P[EPgy2]ClicEY04209 (i.e. ClicEY04209) and P[w[+mC]=lacW]Clic[G0472] (i.e. ClicG0472) were obtained from the Bloomington Drosophila Stock Center (Bloomington, IN, USA). To assess Clic expression, qRT-PCR studies were performed using standard methods as described (Jones et al., 2009). Additional details are provided as Supplementary Material.
