A major potential limitation to the approach used to identify the 51 iMO–human-AUD genes (Table 4) is that it is based on overlap at the level of individual orthologs of iMO and human genes. This approach almost certainly would miss key signaling or biochemical pathways in which, for example, a gene encoding a ligand was investigated in iMOs and the gene encoding the orthologous receptor for that ligand was found to be associated with human AUD. In such a case, the iMO data would provide strong evidence for a role of the biochemical process in alcohol-related behavior relevant to human AUD, even in the absence of directly implicating the particular orthologous human gene. Thus, as a complement to our analysis of the overlap between individual iMO and human genes, we visually compared the predicted or known biochemical functions of the iMO genes (Tables 1 and 2) with the functions of genes described in several comprehensive reviews on the genetics of AUD (Edenberg and Foroud, 2013, 2014; Palmer et al., 2012; Rietschel and Treutlein, 2013; Schuckit, 2014). As is true for
