also upregulated in one or more comparisons; Supplementary Tables 5–8). The high degree of similarity between the four pairwise SILAC analyses (Figure 2; Supplementary Tables 5–8) is clearly evident by gene ontology analysis, which identified oxidative stress/apoptosis and cytoskeletal remodeling pathways in all comparisons (Supplementary Figure 5D). Increased PFN1 (14 kDa) protein was subsequently validated across six controls and four patients by western blot, relative to an actin loading control (Supplementary Figure 5B).
