Many of the most perturbed proteins identified in P1 NPC line A (relative to C1 NPC) were similarly affected in an independent SILAC in comparison with P1 NPC line B (relative to C1 NPC); for example, a known SZ protein, NLGN3, was significantly downregulated in both P1 NPCA and P1 NPC B (Figure 2b). When we subsequently compared a second SZ patient (P3 NPC) with a second and third control (C3 and C6 NPC), we observed several notable changes across independent pairwise comparisons. Three actin-binding proteins involved in cytoskeletal restructuring and migration, profilin (PFN1) and two cofilins (CFL1 and CFL2), were significantly upregulated in SZ in at least three comparisons (Figure 2b). In addition, TXN was significantly upregulated in SZ in three comparisons (Figure 2b) (and many related proteins such as TXNL1, TXNL2, TXNDC1, TXNDC4, TXNDC5, TXNDC12 and TXNRD1 were also upregulated in one or more comparisons; Supplementary Tables 5–8). The high degree of similarity between the four pairwise SILAC analyses (Figure 2; Supplementary Tables 5–8) is clearly evident by gene ontology analysis, which identified oxidative stress/apoptosis and cytoskeletal remodeling
