To test whether the increased alcohol consumption required signaling through the TLR4 system, we deleted CD14, which is a key accessory protein for TLR4 function (Peri et al., 2010). After injection of LPS, these mice also showed some reduction of body weight accompanied by reduction of water intake (Supplemental Figs. 6 and 7), but these reductions were much smaller than were seen in control B6 mice (Fig. 1). Mice lacking CD14 showed no increase of alcohol consumption after pretreatment (Fig. 4). Complete data for ethanol intake (amount of ethanol consumed, preference for ethanol and total fluid intake) in a continuous-access, two-bottle choice test for CD14 null mice of both sexes are presented in Supplemental Figs. 6 and 7 (for detailed statistics see Supplementary materials in Supplemental Table 3).
