The studies presented above used a dose of 1 mg/kg LPS; to determine if a lower dose would increase drinking, 0.33 mg/kg was injected one week before testing drinking. This treatment did not change the ethanol intake, preference for ethanol, or total fluid intake (Supplemental Fig. 5). Because this dose was ineffective for increasing drinking, all subsequent studies were carried out with 1 mg/kg LPS.
