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Chunk #59 — Methods — Flow cytometry analysis

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Inflammasome activity is controlled by ZBTB16-dependent SUMOylation of ASC.
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Single-cell suspensions from peritoneal lavage were stained with APC-CD45, PerCP-Cy5.5-CD11b and PE-Ly6G (BioLegend). Data were acquired on the FACSCanto II (BD Biosciences) and analysed by FlowJo Software.