All experiments were independently performed at least three times in triplicate. Statistical analysis was performed with GraphPad Prism 8.0 software. The statistical difference between the two groups was performed by the unpaired two-tailed t-test or analysis of variance. P values of less than 0.05 were considered significant and different levels of significance were expressed as follows: *p < 0.05; **p < 0.01; ***p < 0.001.
