GTT was performed as described previously62. For NC-fed cohorts, male mice aged 24 weeks (n=5 each group; body weight, 30.93±1.01 g for WT and 29.74±1.26 g for Foxo1 KODAT; P>0.45) were used. For HFD-fed cohorts, male mice fed HFD for 10 weeks (n=9 each group; body weight, 37.94±2.07 g for WT and and 32.74±1.31 g for FoxO1 KODAT; P=0.04) were used. Glucose (1.5 g kg−1 body weight) was injected intraperitoneally.
