For ITT, 24-week old NC-fed male mice (n=5 each group; body weight, 31.22±1.16 g for WT and 29.99±1.26 g for FoxO1 KODAT; P>0.49) or male mice fed HFD for 10 weeks (body weight, 39.64±1.96 g for WT, n=8 and 33.86±1.55 g for FoxO1 KODAT, n=11; P=0.03) were used. Insulin (0.9 U/kg; Eli Lilly and Company, IN, USA) was administered intraperitoneally.
