The rectal body temperature of FoxO1 KODAT and WT littermate mice at the age of 32 weeks for male and at the age of 30 weeks for female (n=5 each group) was measured in a room temperature environment at 9:00 hours by Thermalert TH-5 equipment (Physitemp Instruments, Inc., NJ, USA). Each mouse was measured three times and the average temperature was calculated.